Fluorescent Polymer Microspheres


Fluorescent microspheres emit bright and distinctive colours when illuminated by light of shorter wavelengths than the emission wavelength. This property improves their contrast and visibility relative to background materials. In addition to the features of conventional microspheres, the fluorescent products offer improved sensitivity and detectability for analytical methods.

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This product line was developed for general laboratory use. Applied Microspheres can offer many batches of particles with a wide variety of diameters, that can be custom dyed with different fluorescent colours and intensity levels as required. The dyes are incorporated during the polymerisation process, which prevents dye leakage. Applied Microspheres’ unique technology ensures exceptionally small fluorescence C.V.’s. Dispersion in both aqueous media or air are possible without degradation of their fluorescent properties. They are available as aqueous suspensions or as dry powder. The aqueous medium for the suspensions is specially formulated to prevent the formation of agglomerates and for long-term stability. They can be stored at room temperature.



The fluorescent microspheres can be detected with a flow cytometer, epifluorescence microscope, confocal microscope, fluorescence spectrophotometer, amongst others. They can also be detected using a mineral light or black light and can be observed directly in the matrix or media being tested, or they can be collected on membrane filters for examination by microscope. For some applications, the polymer microspheres can be dissolved and the fluorescent dyes measured directly.

To protect the quality and guarantee the long shelf life of this product:

• Keep the particles in the original opaque bottle to prevent the dye          from photobleaching.
• Keep outside lighting as short as possible.



Typical applications include:
• Flow cytometry instrument calibration and set-up
• Fluorescence microscopy
• Fluorescent instrument monitoring
• Confocal microscopy reference particles
• Filtration media and systems testing
• Vial and container cleaning studies
• Flow tracing and fluid mechanics
• Centrifugation and sedimentation studies


Blue fluorescent polysterene microspheres

Excitation wavelength ranging from 380 – 450 nm.

Emission wavelength ranging from 440 – 520 nm.

1% solids, in aqueous suspension

Dry powder

Green fluorescent polystyrene microspheres



Excitation wavelength ranging from 400 – 490 nm.

Emission wavelength ranging from 470 – 560 nm.

1% solids, in aqueous suspension

Dry powder

Red fluorescent polystyrene microspheres



Excitation wavelength ranging from 450 – 570 nm.

Emission wavelength ranging from 550 – 660 nm.



1% solids, in aqueous suspension

Dry powder